Peptide Mapping

Peptide mapping confirm a protein’s primary structure (amino acid sequence), used in drug discovery and throughout the manufacturing process

Get full sequence of your mAb or therapeutic proteins

  • Protein Digestion

  • Peptide separation

  • Peptide detection

  • Data Interpretation

  • The target protein is enzymatically cleaved into smaller peptides using site-specific proteases like trypsin, which typically cleaves after lysine or arginine residues

  • The resulting peptide mixture is separated using chromatography techniques, such as high-performance liquid chromatography (HPLC), to resolve individual peptides based on their physicochemical properties

  • The separated peptides are analyzed by mass spectrometry (MS) to determine their masses and sequences. Tandem mass spectrometry (MS/MS) is often used to provide detailed sequence information.

  • The MS data is compared to theoretical peptide masses and sequences derived from the known protein sequence. This enables verification of the protein identity and structural features.


Peptide mapping is considered a gold standard in protein characterization, enabling precise structural analysis and ensuring the reliability of therapeutic and research outcomes.

Peptide mapping provides a detailed, high-resolution view of a protein’s composition and structure. This information is critical for:

  • Ensuring the safety, efficacy, and quality of biopharmaceutical products.
  • Supporting regulatory submissions and compliance with guidelines (e.g., FDA, EMA)

 

Protein Identification

Quality Control in Biopharmaceuticals

Post-Translational Modification Analysis

Characterization of Biosimilars