Anaquant HCP analysis I Protein characterisation I Protein analysis
Accurate and reproducible quantification of 90+ CHO Host Cell Proteins using targeted LC-MS/MS and isotope-labelled peptides.
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Accurate and reproducible quantification of 90+ CHO Host Cell Proteins using targeted LC-MS/MS and isotope-labelled peptides.
Coverage of >90 CHO HCPs identified as high-risk
Selection of specific Isotope-labeled peptide standards for each target
Compatible with in-process, intermediate, or drug substance samples
Optimized LC-MS/MS workflow using labeled peptide standards for absolute quantification.
Targeted Quantification of High-Risk HCPs in CHO-Based Biotherapeutics
During bioproduction in CHO cells, residual Host Cell Proteins (HCPs) can remain in the final drug substance despite purification steps. While most HCPs are harmless, a subset of critical or high-risk HCPs may compromise product safety, efficacy, or stability. These include enzymes capable of degrading the therapeutic protein, proteases, lipases and immunogenic contaminants.
ANAQUANT has developed a targeted proteomic method based on stable isotope-labeled peptides to quantify over 90 high-risk CHO HCPs identified from literature and regulatory alerts.
Using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), our method enables precise and multiplexed quantification of the most critical residual proteins in a single analytical run.
Each peptide has been carefully selected and validated to ensure specificity, linearity, and reproducibility, allowing reliable quantification even at trace levels (ng/mg range).
> Evaluate purification efficiency and clearance of high-risk HCPs.
> Monitor consistency across production batches.
> Strengthen your impurity profile with quantitative, validated data.
> Characterize and mitigate critical HCPs that could affect product stability or immunogenicity.